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Key Documents

CBL57

Sigma-Aldrich

Anti-Adrenocorticotropic Hormone Antibody, NT, clone 57

clone 57, Chemicon®, from mouse

Synonym(s):

ACTH

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

100

antibody form

purified antibody

clone

57, monoclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)

isotype

IgG1

NCBI accession no.

NM_000939.2
NM_001035256.1

UniProt accession no.

P01189

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1 of 4

This Item
8.145368.058048.07501
4-Hydroxy-4-methyl-2-pentanone for synthesis

8.04512

4-Hydroxy-4-methyl-2-pentanone

Methyl propyl ketone for synthesis

8.14536

Methyl propyl ketone

4-Methyl-2-pentanol for synthesis

8.05804

4-Methyl-2-pentanol

2-Pentanol for synthesis

8.07501

2-Pentanol

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

density

0.937-0.941 g/cm3 at 20 °C

density

0.81 g/cm3 at 20 °C

density

0.81 g/cm3 at 20 °C

density

0.81 g/cm3 at 20 °C

bp

168 °C/1013 hPa

bp

101 °C/1013 hPa

bp

130-133 °C/1013 hPa

bp

119 °C/1013 hPa

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

mp

-47 °C

mp

-78 °C

mp

-60 °C

mp

-50 °C

Specificity

This antibody is specific for Synacthen (1-24 ACTH) and has no cross reaction with CLIP (ACTH 17-39). The antibody has an affinity constant greater than 10E11 L/mol.

KNOWN SPECIES CROSS REACTIVITY: Positive with rat N-terminal ACTH peptide

Application

This Anti-Adrenocorticotropic Hormone Antibody, N-terminus, clone 57 is validated for use in ELISA, IH(P) for the detection of Adrenocorticotropic Hormone.
This antibody reacts with solid phase ACTH either captured as part of a sandwich or adsorbed as a solid phase antigen and as such is suitable for serum assay development

The antibody is also reactive on formalin fixed paraffin wax embedded tissue and is therefore suitable for immunocyto-chemistry

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: CBL58

Physical form

Format: Purified
The monoclonal is presented as 1mg in 1ml phosphate buffered saline containing 10mM sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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