ABT108

Anti-Lnk/SH2B adapter protein 3 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): SH2B adapter protein 3, Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_005466
• UniProt accession no.: Q9UQQ2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SH2B3(10019)

Description

General description

Lnk is an SH2B3 multidomain adaptor protein that is primarily expressed in lymphocytes. It contains the characteristic pleckstrin homology (PH); src homology 2 (SH2); dimerization; and proline-rich domains. Lnk functions as a negative regulator of B cell proliferative processes via interaction with Jak2 and Mpl proteins, and inhibition of the PI3/Akt/STAT5 signaling pathway. Previous studies have implicated Lnk in celiac disease type 13.

Specificity

Other homologies: Rat (78% sequence homology) and Mouse (72% sequence homology).

This antibody recognizes Lnk/SH2B adapter protein 3 at the C-terminus.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Lnk/SH2B adapter protein 3.

Application

Detect the Lnk/SH2B adapter protein 3 protein using this Anti-Lnk/SH2B adapter protein 3 validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blot in HepG2 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Lnk/SH2B adapter protein 3 on 10 µg of HepG2 cell lysate.

Target description

~80 kDa observed. The calculated molecular weight is 63 kDa, however Lnk/SH2B adapter protein 3 has been shown as a ~80 kDa band in western blots (Li, Y., et al. (2000). J Immunol. 164:5199-5206.)

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HepG2 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable