AB10520

Anti-DAP-5 Antibody

serum, from rabbit

• Synonym(s): Death-associated protein 5, aging-associated protein 1, eIF-4-gamma 2, eIF-4G 2, eIF4G 2, eukaryotic translation initiation factor 4 gamma, 2, eukaryotic translation initiation factor 4G-like 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human
• species reactivity (predicted by homology): rat (based on 100% sequence homology)
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_001036024.1
• UniProt accession no.: P78344
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... EIF4G2(1982)

Description

General description

Death Associated Protein 5 (DAP-5) is a protein with high amino acid sequence homology to Eukaryotic Translation Initiation Factor 4G (eIF4G). Compared with eIF4G, DAP5 lacks the N-terminal region necessary for cap-dependent translation and has a unique C-terminal part functioning as a regulator for interferon-gamma induced cell death. DAP5 is cleaved at Asp790 during apoptosis . The C-terminal truncated form of DAP5 functions as a cap-independent translation initiation factor responsible for the mediation of its own translation during apoptosis.

Specificity

This antibody recognizes DAP-5.

Immunogen

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human DAP-5.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-DAP-5 Antibody is validated for use in WB for the detection of DAP-5.

Quality

Evaluated by Western Blot on Jurkat cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected DAP-5 on 10 µg of jurkat cell lysate.

Target description

97 kDa

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Jurkat cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids