71340-M

pCDFDuet-1 DNA - Novagen

• Synonym(s): Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• UNSPSC Code: 41106602
• NACRES: NA.51

Properties

• form: liquid
• Quality Level: 100
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze
• promoter: Promoter name: T7
• selection: spectinomycin
• storage temp.: −70°C

Description

General description

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Application

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.[1][2][3]

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable