1.05222
GranuCult® plus Kanamycin esculin azide agar
for Enterococcus spp., suitable for microbiology
Synonym(s):
D-Streptococci selective agar
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About This Item
UNSPSC Code:
41106213
NACRES:
NA.74
Recommended Products
Quality Level
sterility
non-sterile
form
granular
packaging
pkg of 500 g
manufacturer/tradename
GranuCult® plus
storage condition
dry media (Keep tightly closed)
technique(s)
microbiological culture: suitable
color
beige
pH
6.9-7.3 (37 °C, 47 g/L in H2O, after autoclaving)
solubility
47.5 g/L
Related Categories
General description
GranuCult® plus Kanamycin esculin azide agar is a highly selective media for D-streptococci. Kanamycin and azide inhibit the accompanying bacterial flora. D-streptococci are, however, only slightly sensitive to these substances, so they grow normally and hydrolyze the glucoside esculin to give glucose and esculetin. Esculetin forms an olive-green to-black complex with iron (III) ions.
Application
GranuCult® plus Kanamycin esculin azide agar is suitable for the isolation, differentiation, and enumeration of enterococci in foodstuffs, water, and other materials.
Features and Benefits
- GranuCult® offers superior granulated culture media
- Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
- Excellent wettability, solubility, and free-flowing properties
- Convenient, with minimal component separation and clumping, even under warm or humid conditions
- High batch-to-batch reproducibility
- Prolonged shelf life of up to five years
- High number of test strains exceeding all regulatory demands
- Granulation technology allows many supplements to be included, with no need to add these separately
Analysis Note
Appearance (clearness): clear
Appearance (colour): brown-bluish
pH-value (25 °C): 6.9 - 7.3
Recovery on test medium (Enterococcus faecalis ATCC 11700): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Staphylococcus aureus ATCC 6538-P (WDCM 00033)): no limit
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Colour change to olivegreen-black (Enterococcus faecalis ATCC 11700): +
Colour change to olivegreen-black (Enterococcus hirae ATCC 8043 (WDCM 00089)): +
Colour change to olivegreen-black (Enterococcus durans ATCC 6056): +
Colour change to olivegreen-black (Staphylococcus aureus ATCC 6538-P (WDCM 00033)): -
Colour change to olivegreen-black (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Colour change to olivegreen-black (Escherichia coli ATCC 11775 (WDCM 00090)): -
Incubation: 48 hrs.; 35 °C; aerobic.
Appearance (colour): brown-bluish
pH-value (25 °C): 6.9 - 7.3
Recovery on test medium (Enterococcus faecalis ATCC 11700): ≥ 70 %
Recovery on test medium (Enterococcus hirae ATCC 8043 (WDCM 00089)): ≥ 70 %
Recovery on test medium (Enterococcus durans ATCC 6056): ≥ 70 %
Recovery on test medium (Staphylococcus aureus ATCC 6538-P (WDCM 00033)): no limit
Recovery on test medium (Bacillus cereus ATCC 11778 (WDCM 00001)): ≤ 0.01 %
Recovery on test medium (Escherichia coli ATCC 11775 (WDCM 00090)): ≤ 0.01 %
Colour change to olivegreen-black (Enterococcus faecalis ATCC 11700): +
Colour change to olivegreen-black (Enterococcus hirae ATCC 8043 (WDCM 00089)): +
Colour change to olivegreen-black (Enterococcus durans ATCC 6056): +
Colour change to olivegreen-black (Staphylococcus aureus ATCC 6538-P (WDCM 00033)): -
Colour change to olivegreen-black (Bacillus cereus ATCC 11778 (WDCM 00001)): -
Colour change to olivegreen-black (Escherichia coli ATCC 11775 (WDCM 00090)): -
Incubation: 48 hrs.; 35 °C; aerobic.
Footnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Certificates of Analysis (COA)
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