ZRB2902

Anti-CHD2 Antibody, clone 2J13 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): ATP-dependent helicase CHD2, Chromodomain-helicase-DNA-binding protein 2
• UNSPSC Code: 12352203

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2J13, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 211.34 kDa; observed mol wt ~211 kDa
• purified by: using Protein A
• species reactivity: human
• species reactivity (predicted by homology): rat, mouse
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001262.3
• UniProt accession no.: O14647
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... CHD2(1106)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2J13 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects CHD2. It targets an epitope within 25 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human CHD2.

Application

Quality Control Testing

Evaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CHD2 in HaCaT cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected CHD2 in lysate from HEK-293 cells nuclear extract.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CHD2 in SH-SY5Y cells.

Affinity Binding Assay: A representative lot of this antibody bound with CHD2 peptide at least one thousand-fold (1,000X) higher affinity than with non-specific control peptide.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CHD2 in human cerebral cortex tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Chromodomain-helicase-DNA-binding protein 2 (UniProt: O14647; EC:3.6.4.12; also known as ATP-dependent helicase CHD2) is encoded by the CHD2 gene (Gene ID: 1106) in human. CHD2 is a member of the CHD (chromodomain helicase DNA-binding) family of proteins, characterized by the presence of multiple domains, including two chromodomains, a helicase domain, and an SNF2-related helicase/ATPase domains. CHD2 is primarily involved in chromatin remodeling and regulation of gene expression. It plays a crucial role in DNA repair, transcriptional regulation, and maintaining genomic stability. CHD2 is known to facilitate the assembly of chromatin complexes and is involved in the recruitment of other proteins necessary for DNA damage response. Dysregulation of CHD2 has been implicated in several diseases, including various cancers and neurological disorders. Mutations or altered expression of CHD2 can lead to genomic instability, contributing to tumorigenesis. In neurological contexts, CHD2 mutations have been associated with epilepsy and other neurodevelopmental disorders, highlighting its critical role in brain function and development. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable