ZRB2640

Anti-TMEM106B Antibody, clone 2E4 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Transmembrane protein 106B
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 2E4, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 31.13 kDa; observed mol wt ~42 kDa
• purified by: using Protein A
• species reactivity: rat, human
• species reactivity (predicted by homology): monkey
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal cytoplasmic domain
• Protein ID accession no.: NP_001127704
• UniProt accession no.: Q9NUM4
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... TMEM106B(54664)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2E4 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Transmembrane protein 106B (TMEM106B). It targets an epitope within 22 amino acids from the N-terminal, cytoplasmic domain.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the N-terminal, cytoplasmic domain of human Transmembrane protein 106B (TMEM106B).

Application

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM106B in Jurkat cell lysate.

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound TMEM106B peptide with a KD of 1.1 x 10-7 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TMEM106B in Human testis and Rat brain tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMEM106B in U2OS cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Transmembrane protein 106B (UniProt: Q9NUM4; also known as TMEM106B) is encoded by the TMEM106B gene (Gene ID: 54664) in human. TMEM106B is a single-pass type II membrane protein that contains a cytoplasmic domain (aa 2-96), a transmembrane domain (aa 97-117), and a lumenal domain (aa 118-274). Its expression is observed in the brain, including the frontal cortex. Some expression has also been reported in lung epithelial cells. In neurons, it is involved in the transport of late endosomes/lysosomes and may also be involved in dendrite morphogenesis and maintenance by regulating lysosomal trafficking. It may serve as a molecular brake for retrograde transport of late endosomes/lysosomes, possibly via its interaction with MAP6. It also plays a role in the regulation of lysosomal size and responsiveness to stress and is required for lysosomal acidification. A truncated C-terminal fragment of TMEM106B (aa 120-354) has also been reported that aggregates into stable amyloid fibrils in the brain of subjects suffering from diverse genetic and sporadic tauopathies, amyloid-β amyloidoses, and synucleinopathies. It also plays a role in ACE2-independent SARS-CoV-2 entry into cell where it is required for post-endocytic stage of virus entry by facilitating spike-mediated membrane fusion. Mutations in TMEM106B gene have been linked to Ubiquitin-positive frontotemporal dementia (UP-FTD), an autosomal dominant neurodegenerative disease. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Baggen, J., et al. (2023). Cell. 186(16); 3427-3442; Stagi, M., et al. (2014). Mol. Cell Neurosci. 61: 226-240).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable