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SRP0325

Sigma-Aldrich

PHF8 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE)

Synonym(s):

JHDM1F, MRXSSD, PHD finger protein 8, ZNF422

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected Sf9 cells

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

52 kDa

packaging

pkg of 20 μg

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PHF8(23133)

General description

Human PHF8 (also known as JHDM1F), (GenBank Accession No. NM_001184896), amino acids 2 - 450 with N-terminal FLAG-tag, MW=52 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physical form

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/mL Flag peptide, 20% Glycerol, and 3 mM DTT.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Wen Liu et al.
Nature, 466(7305), 508-512 (2010-07-14)
While reversible histone modifications are linked to an ever-expanding range of biological functions, the demethylases for histone H4 lysine 20 and their potential regulatory roles remain unknown. Here we report that the PHD and Jumonji C (JmjC) domain-containing protein, PHF8

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