SRP0220

DEP1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): CD148, DEP-1, Density-enhanced phosphatase 1, PTPRJ, Protein tyrosine phosphatase, receptor type, J
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• potency: ≥0.114 units/mg
• mol wt: 58.9 kDa
• packaging: pkg of 20 μg
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_U10886
• UniProt accession no.: Q12913
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PTPRJ(5795)

Description

General description

Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system.

Application

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.