SRP0189

Pyruvate kinase M2 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

• Synonym(s): M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3), Pyruvate kinase, muscle, Thyroid hormone-binding protein 1 (THBP1)
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• biological source: mouse
• recombinant: expressed in E. coli
• Assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 58 kDa
• packaging: pkg of 20 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: >0.02 mg/mL
• NCBI accession no.: NM_011099
• UniProt accession no.: P52480

Description

General description

Pyruvate kinase M2 (PKM2), also known as pyruvate kinase 3 (PK3), is an isoform of pyruvate kinase that is expressed in some differentiated tissues, such as lung, fat, retina, and pancreatic islets, as well as in normal proliferating cells and embryonic cells.[1] PKM2/pk3 is encoded by the gene mapped to mouse chromosome 9.[2]
Murine Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_011099), amino acids 2-end, with N-terminal His tag, MW = 58kDa, expressed in an Escherichia coli expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Elevated expression of PKM2 is observed in most of the cancer cells. Hence, this protein can be considered as a potent therapeutic target for cancers.[3][1] PKM2 plays an important role in the discharge of excessive intracellular reactive oxygen species (ROS), which otherwise damage cellular components and decrease cell viability. Protein kinase activity of PKM2 is involved in the regulation of gene expression and tumorigenesis. PKM2 is an important rate-limiting enzyme involved in final step of glycolysis and it has an essential role in metabolic reprogramming throughout cancer progression.[1][3]

Unit Definition

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.