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SH2921

Sigma-Aldrich

MISSION® shRNA Human Gene Family Set, DNA

B-Cell Activation

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About This Item

UNSPSC Code:
41106609
NACRES:
NA.51
Pricing and availability is not currently available.

product line

MISSION®

storage temp.

−20°C

Gene Information

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1 of 4

This Item
SML1741T7665SML0688
UNC10201652 ≥98 mg/mL (HPLC)

SML3236

UNC10201652

TH1020 ≥95% (HPLC)

SML1741

TH1020

Tyrphostin 51 ≥98%

T7665

Tyrphostin 51

PO1 ≥98% (HPLC)

SML0688

PO1

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

100

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

solubility

DMSO: 2 mg/mL, clear

solubility

DMSO: 3 mg/mL, clear (warmed)

solubility

DMSO: soluble 5 mg of Tyrphostin 51 in 0.1 ml of solvent, clear, orange to red

solubility

DMSO: 1 mg/mL, clear (warmed)

color

white to beige

color

white to beige

color

-

color

faintly pink to red

concentration

≥98 mg/mL (HPLC)

concentration

-

concentration

-

concentration

-

General description

Sequenced-verified shRNA lentiviral plasmids (pLKO.1-puro) are provided as purified plasmid DNA suitable for virus production and transient transfection. DNA is provided in 40 μl aliquots per well in Tris,EDTA (TE) solution. An average of 2 μg per clone is provided per well with a range from 400 ng to 4 μg. The set comes in 96-well plates that are barcoded for simple identification. A CD containing RefSeq, gene description, gene symbol, clone ID, hairpin sequence, locus link, and plate map positions are provided with the gene family set.

Other Notes

Each MISSION shRNA clone is constructed within the lentivirus plasmid vector, pLKO.1-Puro, followed by transformation into Escherichia coli. The pLKO.1-Puro vector contains bacterial (ampicillin) and mammalian (puromycin) antibiotic resistance genes for selection of inserts in either bacterial or mammalian cell lines. Each clone set consists of an average of 3-5 constructs that have been designed against each target gene using a proprietary algorithm. Therefore, a range of knockdown efficiency, with at least one construct from each gene set being >70%, can be expected when using these clones. This allows one to examine the effect of loss of gene function over a large series of gene knockdown efficiencies. Each shRNA construct has been cloned and sequence verified to ensure a match to the target gene.
For a detailed listing of other available gene family sets, visit the gene family set website.
Number of Genes: 99, Number of Clones: 661
The exact gene and clone count at time of purchase may vary slightly as the TRC library is continually updated.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense .
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Pierre Youinou
    Journal of autoimmunity, 28(2-3), 143-151 (2007-03-17)
    The interest for B cells has recently been revived. They normally play a role in the development, the regulation, as well as the activation of lymphoid architecture: they regulate dendritic cells and T-cell subsets function through cytokine production. Receptor editing
    Pavel Tolar et al.
    Immunological reviews, 221, 64-76 (2008-02-16)
    The binding of antigen to the B-cell receptor (BCR) induces BCR clustering and signaling cascades that lead to the activation of a variety of genes associated with B-cell activation. Over the last several years, our understanding of the molecular details

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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