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M1254

Sigma-Aldrich

MOPS

≥99.5% (titration), crystalline powder

Synonym(s):

3-(N-Morpholino)propanesulfonic acid, 4-Morpholinepropanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C7H15NO4S
CAS Number:
Molecular Weight:
209.26
Beilstein:
1106776
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Product Name

MOPS, ≥99.5% (titration)

Quality Level

Assay

≥99.5% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

affinity chromatography: suitable
electrophoresis: suitable

color

white

pH

2.5-4 (25 °C, 209 g/L)

useful pH range

6.5-7.9

pKa (25 °C)

7.2

solubility

water: 0.5 g/mL, clear, colorless

λ

33 % in H2O

suitability

suitable for HPLC

application(s)

clinical research
diagnostic assay manufacturing
life science and biopharma

storage temp.

room temp

SMILES string

OS(=O)(=O)CCCN1CCOCC1

InChI

1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)

InChI key

DVLFYONBTKHTER-UHFFFAOYSA-N

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General description

MOPS (3-(N-morpholino)propanesulfonic acid) also known as Propane Sultone buffer, is a versatile "Good′s buffer" extensively employed in biological research. It shares structural similarities with MES (2-(N-morpholino)ethanesulfonic acid), featuring a morpholine ring. In terms of physiological relevance, MOPS′ pKa of 7.2 closely aligns with physiological pH, making it suitable for studies requiring a near-neutral environment. Compared to MES with a pKa of 6.1, MOPS offers a more physiologically relevant buffering capacity.

MOPS serves various functions in diverse research domains. As a buffering agent, it can be used to stabilize pH in cell culture media, promoting optimal cell growth and function. Additionally, it may find application in protein purification in chromatography, stabilizes protein solutions, and acts as a reliable running buffer in various electrophoretic techniques, enabling efficient separation and analysis of biomolecules. MOPS also efficiently lyses cells for subsequent protein or nucleic acid extraction and stabilizes enzymes in solution, ensuring their activity for research purposes. It further proves valuable in fine-tuning the pH of growth media for optimal cell growth.

MOPS possesses additional advantages such as minimal interaction with metal ions especially with copper (Cu), nickel (Ni), manganese (Mn), zinc (Zn), cobalt (Co) ions, making it ideal for studies involving metal ions. It exhibits excellent water solubility, facilitating its use in various buffer formulations, and minimal lipid solubility, ensuring impermeability to membranes and minimizing interference with cellular processes. In summary, MOPS is a reliable buffer widely utilized across diverse biological disciplines, distinguished by its versatility, physiological relevance, minimal metal interaction, and ease of use, with its extensive applications in cell biology, protein studies, electrophoresis, and biochemical research.

Application

MOPS has been used as:

  • a cell culture additive component in lentiviral particle production
  • as a buffering agent in microbial growth medium and nuclei extraction buffer
  • as a component of the MMSE-A buffer for isolating heart mitochondria
  • as a component of the Synthetic Complete (SC) medium
  • as a buffer in fluorescence quenching of NADH

Features and Benefits

  • Ideal for Cell Biology and Biochemical research
  • Can be used as a Buffer component for Electrophoresis and Protein separation
  • Effective Buffering from pH 6.5-7.9 (25 °C) with a pKa of 7.2 (25 °C)
  • Highly soluble in water
  • Minimal metal ion binding

Packaging

The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR®packaging:
  • The current DRYPOUR®packaging configuration is in a 50L nestable drum
  • 25KG-DP are the standard prepack offering
  • Smaller volumes can be put in the same drum for variable configurations
The benefits of DRYPOUR®:
  • Improves operational efficiency by reducing material preparation time and increasing operator safety
  • Minimizes caking of hygroscopic salts
  • Offers triple protection: against moisture inside and outside, and against contamination
How does the DRYPOUR®packaging system Improve your operational efficiency?
  • This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
  • PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek®liner
  • Desiccant bags are firmly integrated into the polyethylene liner
  • Triple protection delivers two invaluable results:
  1. Dramatically reduced caking
  2. No contamination risk from the desiccant

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

230.0 °F - closed cup

Flash Point(C)

110 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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