I7908

IL-2 human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in Pichia pastoris, lyophiilized powder, suitable for cell culture

• Synonym(s): IL-2, Interleukin-2, T-cell growth factor
• MDL number: MFCD00165721
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• Product Name: Interleukin-2 human, recombinant, expressed in Pichia pastoris, suitable for cell culture
• biological source: human
• Quality Level: 200
• recombinant: expressed in Pichia pastoris
• Assay: ≥98% (SDS-PAGE and HPLC)
• form: lyophilized powder
• mol wt: predicted mol wt ~15 kDa
• packaging: pkg of 10KU
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested

Description

Application

Interleukin-2 human has been used to assess changes in tumor necrosis factor receptor 1 (TNFR1). It has also been used in TNFR2 expression on Tregs.[1]

Biochem/physiol Actions

Interleukin 2 (IL2) is a pro-inflammatory cytokine.[2] It consists of three subunits, in which the β subunit is important for receptor mediated signaling.[3] IL2 enhances the development of T regulatory cells[4] and plays a crucial role in antitumor immunity.[2]

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Physical form

Lyophilized from a 0.2 μm filtered solution in 50 mM sodium phosphate buffer, pH 7.0, containing 0.1% human serum albumin per 0.1 mL of cytokine.

Analysis Note

The biological activity is tested in a cell proliferation assay using an IL-2 dependent murine cytotoxic T cell line, CTLL-2.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves