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Key Documents

HPA003578

Sigma-Aldrich

Anti-ARHGEF6 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-α-Pix antibody produced in rabbit, Anti-ρ-Guanine nucleotide exchange factor 6 antibody produced in rabbit, Anti-COOL-2 antibody produced in rabbit, Anti-PAK-interacting exchange factor α antibody produced in rabbit, Anti-Rac/Cdc42 guanine nucleotide exchange factor 6 antibody produced in rabbit

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
HPA003578
NACRES:
NA.41

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:50-1:200

immunogen sequence

VQYGACEEKEERYLMLFSNVLIMLSASPRMSGFIYQGKIPIAGTVVTRLDEIEGNDCTFEITGNTVERIVVHCNNNQDFQEWLEQLNRLIRGPASCSSLSKTSSSSCSAHS

UniProt accession no.

Q15052

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ARHGEF6(9459)

General description

The ARHGEF6 (Rac/Cdc42 guanine nucleotide exchange factor (GEF) 6), also referred to as PIXA, is mapped to human chromosome Xq26. The encoded protein is a member of the Dbl-related guanine nucleotide exchange factor (GEF) protein family.

Immunogen

ρ-Guanine nucleotide exchange factor 6 recombinant protein epitope signature tag (PrEST)

Application

Anti-ARHGEF6 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Biochem/physiol Actions

Rho guanine nucleotide exchange factor 6 plays a crucial role in BαP-induced apoptosis by activation of the c-Jun NH2-terminal kinase 1 (JNK1) pathway kinases. It is responsible for Börjeson-Forssman-Lehmann syndrome (BFLS) or MRX27 and is a prime candidate for nonspecific or syndromic mental retardation linked to Xq26. The encoded protein catalyzes the exchange of GDP for GTP and enhances the activity of the Rho-GTPases Rac1 and Cdc42. It is found to be involved in the regulation of cell adhesion and motility, chemotaxis, neuronal morphogenesis and function, and receptor-mediated signaling events. α-PIX forms a complex with E3 ubiquitin ligase c-Cbl and regulates EGFR (epidermal growth factor receptor) trafficking.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST74378

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
R00537
A08318
R000537

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K M Lower et al.
American journal of medical genetics, 100(1), 43-48 (2001-05-05)
Börjeson-Forssman-Lehmann syndrome (BFLS) is a syndromic X-linked mental retardation that has been mapped by linkage to Xq26-q27. A nonsyndromic mental retardation family, MRX27, has also been localized to a region of the X chromosome overlapping Xq26-q27. The gene for ARHGEF6
S Yoshii et al.
Molecular and cellular biology, 21(20), 6796-6807 (2001-09-21)
Benzo[a]pyrene [B(a)P], a potent procarcinogen found in combustion products such as diesel exhaust and cigarette smoke, has been recently shown to activate the c-Jun NH(2)-terminal kinase 1 (JNK1) and induce caspase-3-mediated apoptosis in Hepa1c1c7 cells. However, the molecules of the
Fanny Kortüm et al.
PloS one, 10(7), e0132737-e0132737 (2015-07-16)
Endosomal sorting is an essential control mechanism for signaling through the epidermal growth factor receptor (EGFR). We report here that the guanine nucleotide exchange factor αPIX, which modulates the activity of Rho-GTPases, is a potent bimodal regulator of EGFR trafficking.

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