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H7006

Sigma-Aldrich

HEPES sodium salt

≥99.0% (titration)

Synonym(s):

Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt

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About This Item

Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99.0% (titration)

form

powder

storage condition

dry at room temperature

technique(s)

HPLC: suitable

color

white

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

suitability

suitable for mass spectrometry

application(s)

diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics

storage temp.

room temp

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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General description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Application

HEPES buffer finds application in:
  • Most studies with metal ions
  • Substitute for Tris and phosphate in studies with metal ions
  • Grinding buffer in plant studies
  • Running buffer in gel electrophoresis
  • Buffer in electroporation studies
  • Bradford or bicinchoninic acid (BCA) assays
  • Extraction buffer to prevent damage of proteins in red blood cells

It has been used:
  • as a buffer component in the preparation of a low sucrose buffer
  • as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

Features and Benefits

  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water
  • Negligible metal ion binding
  • Less toxic to cells than other buffers such as Tris and phosphate
  • Stable in a wide pH range

Packaging

The DRYPOUR®packaging system for high-quality hygroscopic salts DRYPOUR® packaging:
  • The current DRYPOUR® packaging configuration is in a 50L nestable drum
  • 25KG-DP are the standard prepack offering
  • Smaller volumes can be put in the same drum for variable configurations
The benefits of DRYPOUR®:
  • Improves operational efficiency by reducing material preparation time and increasing operator safety
  • Minimizes caking of hygroscopic salts
  • Offers triple protection: against moisture inside and outside, and against contamination
How does the DRYPOUR®packaging system Improve your operational efficiency?
  • This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
  • PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek® liner
  • Desiccant bags are firmly integrated into the polyethylene liner
  • Triple protection delivers two invaluable results:
  1. Dramatically reduced caking
  2. No contamination risk from the desiccant

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

DRYPOUR is a registered trademark of Merck KGaA, Darmstadt, Germany
Tyvek is a registered trademark of E. I. du Pont de Nemours and Company

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Nishant Mehta et al.
Cell reports, 28(10), 2509-2516 (2019-09-05)
V-domain immunoglobulin (Ig) suppressor of T cell activation (VISTA) is an immune checkpoint protein that inhibits the T cell response against cancer. Similar to PD-1 and CTLA-4, a blockade of VISTA promotes tumor clearance by the immune system. Here, we report a
Åsa Birna Birgisdottir et al.
Autophagy, 15(8), 1333-1355 (2019-02-16)
Autophagosome formation depends on a carefully orchestrated interplay between membrane-associated protein complexes. Initiation of macroautophagy/autophagy is mediated by the ULK1 (unc-51 like autophagy activating kinase 1) protein kinase complex and the autophagy-specific class III phosphatidylinositol 3-kinase complex I (PtdIns3K-C1). The
Rakesh K Pathak et al.
Analytical chemistry, 84(11), 5117-5123 (2012-05-04)
An in situ prepared Zn(2+) complex of triazole linked imino-thiophenyl conjugate of calix[4]arene, [ZnL], was demonstrated to be highly fluorescent in HEPES buffer solution. [ZnL] has been used as a chemo-sensing ensemble for the recognition of phosphates in general and
Jemimah Walker et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 100(4), 1134-1141 (2012-02-15)
Magnesium (Mg) and its alloys have been proposed as degradable replacements to commonly used orthopedic biomaterials such as titanium alloys and stainless steel. However, the corrosion of Mg in a physiological environment remains a difficult characteristic to accurately assess with
Junbo Li et al.
Dalton transactions (Cambridge, England : 2003), 41(13), 3623-3626 (2012-02-24)
We developed a novel method for Cu(2+) detection based on the ion promoted oxidation reaction. Chemodosimeter L (weak fluorescence) can be oxidized into 3-benzothiazoly-7-N,N-diethylaminocoumarin (strong green fluorescence, coumarin 6) by Cu(2+) with high selectivity and sensitivity in HEPES (10 mM

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