H7006
HEPES sodium salt
≥99.0% (titration)
Synonym(s):
Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
About This Item
Recommended Products
Quality Level
Assay
≥99.0% (titration)
form
powder
storage condition
dry at room temperature
technique(s)
HPLC: suitable
color
white
pH
10-12 (1 M in water)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
water: 0.1 M, clear, colorless
suitability
suitable for mass spectrometry
application(s)
diagnostic assay manufacturing
general analytical (
)
life science and biopharma
metabolomics
storage temp.
room temp
SMILES string
[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O
InChI
1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1
InChI key
RDZTWEVXRGYCFV-UHFFFAOYSA-M
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General description
Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.
Application
- Most studies with metal ions
- Substitute for Tris and phosphate in studies with metal ions
- Grinding buffer in plant studies
- Running buffer in gel electrophoresis
- Buffer in electroporation studies
- Bradford or bicinchoninic acid (BCA) assays
- Extraction buffer to prevent damage of proteins in red blood cells
It has been used:
- as a buffer component in the preparation of a low sucrose buffer
- as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions
Features and Benefits
- Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
- Highly soluble in water
- Negligible metal ion binding
- Less toxic to cells than other buffers such as Tris and phosphate
- Stable in a wide pH range
Packaging
- The current DRYPOUR® packaging configuration is in a 50L nestable drum
- 25KG-DP are the standard prepack offering
- Smaller volumes can be put in the same drum for variable configurations
- Improves operational efficiency by reducing material preparation time and increasing operator safety
- Minimizes caking of hygroscopic salts
- Offers triple protection: against moisture inside and outside, and against contamination
- This state-of-the-art design packaging system minimizes caking, thus significantly reducing the time needed for material preparation
- PE drum with our new tamper-evident seal, a polyethylene liner with integrated desiccant bags and a breathable interior Tyvek® liner
- Desiccant bags are firmly integrated into the polyethylene liner
- Triple protection delivers two invaluable results:
- Dramatically reduced caking
- No contamination risk from the desiccant
Physical form
Other Notes
Legal Information
comparable product
related product
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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