CCRF S-180 II
90011901, mouse unknown/unspecified, Fibroblast
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About This Item
UNSPSC Code:
41106514
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Product Name
CCRF S-180 II, 90011901
biological source
mouse unknown/unspecified
packaging
tube of 5 μg 90011901-DNA-5UG
pkg of vial of cells 90011901-1VL
growth mode
Adherent
karyotype
2n = 40
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
Cell Line Origin
Mouse CFW sarcoma 180
Cell Line Description
Established from an S-180 sarcoma maintained in adult CFW mice. The cells have been used extensively in chemotherapy screening tests.
Application
Chemotherapy screening tests
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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