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Key Documents

LIM2551

12070505, human colon, epithelial; forms aggregates of small rounded cells, adherent and suspended.

Synonym(s):

LIM 2551, LIM-2551

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About This Item

UNSPSC Code:
41106514

Product Name

LIM2551, 12070505

biological source

human colon

packaging

tube of 5 μg 12070505-DNA-5UG
pkg of vial of cells 12070505-1VL

growth mode

Semi-adherent

karyotype

Not specified

morphology

Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.

receptors

Not specified

relevant disease(s)

cancer

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Cell Line Origin

Human derived from an adenocarcinoma of the transverse colon

Cell Line Description

LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.

DNA Profile

Not specified

Culture Medium

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.

Subculture Routine

Optimal split ratio 1:8-1:16 (seeding density 1 x 104 cells/cm2). Cells incubated at 37 °C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia

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