AB5405

Anti-Opsin Antibody, Red/Green

Chemicon^®, from rabbit

• Synonym(s): Anti-CBBM, Anti-CBP, Anti-COD5, Anti-RCP, Anti-ROP
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin)
• NCBI accession no.: NM_020061.3
• UniProt accession no.: P04000

Description

General description

Long-wave-sensitive opsin 1/Medium-wave-sensitive opsin 1 (UniProt: P04000/P04001; also known as Red cone photoreceptor pigment/Green cone photoreceptor pigment, Red-sensitive opsin/ Green-sensitive opsin, ROP/GOP) are encoded by the OPN1LW/OPN1MW (also known as RCP/GCP) genes (Gene ID: 5956/2652) in human. The full range of color discrimination in humans is based on the presence and function of three cone photoreceptors. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein that gives sensitivity in the short (S cone, peak sensitivity about 420 nm), middle (M cone, peak sensitivity about 530 nm with polymorphism), and long (L cone, peak sensitivity about 560 nm with polymorphism) wavelengths of the light spectrum. Opsins are multi-pass membrane proteins that belongs to the G-protein coupled receptor 1 family. They consist of four extracellular, 7 helical, and four cytoplasmic domains. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event. The S cone (blue) opsin seems to have a stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments. Mutations in OPN1MW and OPN1LW genes are known to cause color blindness that is characterized by a dichromasy in which red and green are confused, without loss of luminance or shift or shortening of the spectrum. Some mutations also lead to cone dystrophy leading to progressive degeneration of the cone photoreceptor with some preservation of rod function. (Ref.: Neitz, M., and Neitz, J. (2000). Arch. Ophthalmol. 118(5); 691-700).

Specificity

Mouse. Reactivity with other species has not been determined

This rabbit polyclonal antibody detects Red-sensitive opsin/ Green-sensitive opsins.

Immunogen

Full-length, recombinant human red/green opsin.

Recombinant human red/green opsin.

Application

Anti-Opsin Red/Green, Cat. No. AB5405, is a rabbit polyclonal antibody that detects Opsin Red/Green and is tested for use in Immunohistochemistry (Paraffin).

Quality

Evaluated by Immunohistochemistry (Paraffin) in Mouse retina tissue sections.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Opsin Red/Green in Mouse retina tissue sections.

Physical form

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable