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Merck

T2694

Sigma-Aldrich

Trizma® hydrochloride solution

pH 8.0, BioPerformance Certified, 1 M, suitable for cell culture

Synonym(s):

Tris hydrochloride solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25
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grade

BioPerformance Certified
Molecular Biology

Quality Level

form

solution

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

DNase, RNase, Protease, none detected
bioburden, tested
endotoxin, tested
≤5 ppm Heavy metals (as Pb)

pH

8.0

useful pH range

7.0-9.0

absorption

≤0.05 at 290 at 40%

General description

A series of Pre-mixed solutions of TRIZMA Base and TRIZMA HCl to provide commonly used pH values for Tris buffers. No mixing or pH adjustment necessary. Guaranteed accuracy ± 0.1 pH units.

Application

Trizma® hydrochloride solution has been used:
  • as a component of extraction buffer for Plasmodiophora brassicae DNA isolation
  • as a component of resuspension buffer for human genomic DNA fragmented sample
  • for adjusting pH of plasma samples and as a component of reconstitution and neutralization buffer

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany


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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves


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