298711

Diethyl Pyrocarbonate

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca²⁺ permeability of SR vesicles.

• Synonym(s): Diethyl Pyrocarbonate, Diethyl Oxydiformate, DEPC
• Empirical Formula (Hill Notation): C₆H₁₀O₅
• CAS Number: 1609-47-8
• Molecular Weight: 162.14
• MDL number: MFCD00009106
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• Quality Level: 100
• description: Merck USA index - 14, 7998
• Assay: ≥97% (titration)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: clear colorless
• solubility: chloroform: soluble; ethanol: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• SMILES string: O(CC)C(=O)OC(=O)OCC
• InChI: 1S/C6H10O5/c1-3-9-5(7)11-6(8)10-4-2/h3-4H2,1-2H3
• InChI key: FFYPMLJYZAEMQB-UHFFFAOYSA-N

Description

General description

Inhibits ryanodine binding to ryanodine/Ca²⁺ receptor channel in skeletal muscle in a dose- and time-dependent manner and increases the Ca²⁺ permeability of SR vesicles. Useful for specific inactivation of nucleases during isolation of undegraded polynucleotides. Also inhibits platelet-activating factor acetyl hydrolase. Has been used as a probe for the topography of 5.8S rRNA in yeast ribosomes.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Inhibits ryanodine binding to ryanodine/Ca2+ receptor channel in skeletal muscle

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Harmful (C)

Other Notes

Shoshan-Barmatz, V., and Weil, S. 1994. Biochem. J. 299, 177.
Blumbers, D.O. 1987. Methods Enzymol. 152, 20.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Precautionary Statements: P264 - P270 - P301 + P312 - P501
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): 156.2 °F - closed cup
• Flash Point(C): 69 °C - closed cup