SRE0005

Proteinase K from Tritirachium album

Name: Proteinase K from <I>Tritirachium album</I>
Brand: SIGMA

Reagents designed and manufactured under current ISO 13485 certification.

Synonim(y):

Endopeptidase K

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About This Item

CAS Number:

39450-01-6

Numer WE:

3.4.21.64 (BRENDA, IUBMB)

Numer WE:

254-457-8

Numer MDL:

MFCD00132129

Kod UNSPSC:

12352204

NACRES:

NA.32

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Pomoc techniczna

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Pozwól nam pomóc

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Pozwól nam pomóc

klasa czystości

Molecular Biology

Poziom jakości

500

Formularz

buffered aqueous glycerol solution

masa cząsteczkowa

28.93 kDa

stężenie

≥10 mg/mL protein
≥800 unit/mL

zanieczyszczenia

≤500 pg/mg DNA (PicoGreen^® assay)

Zastosowanie

diagnostic assay manufacturing

obecność zanieczyszczeń

DNAse, RNAse, exonuclease, endonuclease, and nickase, none detected

Warunki transportu

wet ice

temp. przechowywania

2-8°C

Zastosowanie

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Działania biochem./fizjol.

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Definicja jednostki

One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).