R6148

RNase A solution

• Synonim(y): 904 RNAza A
• Kod UNSPSC: 12352204
• NACRES: NA.52

Właściwości

• pochodzenie biologiczne: bovine pancreas
• Poziom jakości: 200
• Formularz: liquid
• aktywność właściwa: 1500 units/mL; 3000 units/mL
• stężenie: 20 mg/mL in Tris buffer
• metody: DNA purification: suitable
• temp. przechowywania: room temp
• informacje o genach: bovine ... RNASE1(282340), RNASE1(280930)

Opis

Opis ogólny

Ribonuclease A (RNase A), a protein composed of three α helices and seven β strands arranged in two "lobes” holds significance as the prototypical domain-swapped protein. It is known for its diverse oligomeric states. The RNase A family derives its name from bovine pancreatic RNase A and is specific to vertebrates. In humans, this family encompasses eight functional members, showcasing its biological relevance.
For sample preparation in GenElute^™ kits.

Zastosowanie

RNase A solution has been used:

• in apoptosis assay of Hep-2 cells
• for the colorimetric quantification of methylated DNA
• for DNA and RNA extraction
• in DNA laddering analysis
• in cell cycle analysis
• as a component in phosphate-buffered saline (PBS) to incubate cells for flow cytometry

Działania biochem./fizjol.

Ribonuclease A or bovine pancreatic ribonuclease A (RNase A) enzyme is an endoribonuclease that displays a specific ability to cleave single-stranded RNA on the 3′ side of pyrimidine residues. This endonuclease with 124 amino acids plays a key role in gene duplication, cellular metabolism, and cancer therapy. Its overexpression is closely associated with several types of cancer. Hence, RNase A has the potential to be a promising new biomarker for the diagnosis of malignant tumors and is also used as a specific target for new drug discovery. RNase A is commonly used for the isolation of plasmid DNA.

Uwaga dotycząca przygotowania

For usage requirements, please refer to the kit procedure.

Informacje prawne

GenElute is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable