P8340

Protease Inhibitor Cocktail

Name: Protease Inhibitor Cocktail
Brand: SIGMA

DMSO solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, DMSO solution

Synonim(y):

Mieszanka inhibitorów proteazy

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About This Item

Numer MDL:

MFCD00677817

Kod UNSPSC:

12352200

NACRES:

NA.77

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Product Name

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

pochodzenie biologiczne

bovine lung

Poziom jakości

300

Formularz

DMSO solution

rozpuszczalność

water: soluble

temp. przechowywania

−20°C

Opis ogólny

Protease Inhibitor Cocktail for mammalian cell and tissue extracts, in DMSO solution. It contains six individual components for inhibiting serine, cysteine, and acid proteases, and aminopeptidases.

Specyficzność

Inhibits serine, cysteine, and acid proteases, and aminopeptidases.

Zastosowanie

Ideal for use as a buffer component during sonication, lysis buffer, and RIPA buffer

Provides protection against endogenous enzyme activity in cell and tissue extracts

Działania biochem./fizjol.

This mixture contains individual components, including AEBSF at 104 mM, Aprotinin at 80 μM, Bestatin at 4 mM, E-64 at 1.4 mM, Leupeptin at 2 mM and Pepstatin A at 1.5 mM. Each component has specific inhibitory properties. AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. Leupeptin acts against both serine and cystein proteases. Pepstatin A inhibits acid proteases.

Cechy i korzyści

Broad-spectrum inhibition: inhibits multiple classes of proteases and aminopeptidases

Stable storage: supplied as a solution in DMSO, allowing for long-term storage at -20°C

Consistent performance: ready-to-use solution

Komponenty

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Przestroga

This cocktail should be stored at -20 °C.

Uwaga dotycząca przygotowania

One mL of this cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g of bovine liver or 10 mL of lysate from CHO cells. This product is supplied as a solution in DMSO.