P8215

Protease Inhibitor Cocktail

Name: Protease Inhibitor Cocktail
Brand: SIGMA

DMSO solution, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use with fungal and yeast extracts, DMSO solution

Synonim(y):

Inhibitor proteazy

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych

About This Item

Numer WE:

200-664-3

Numer MDL:

MFCD00677817

Kod UNSPSC:

12352200

NACRES:

NA.54

Kluczowe dokumenty

Wyświetl całą dokumentację

Pomoc techniczna

Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.

Pozwól nam pomóc

Pomoc techniczna

Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.

Pozwól nam pomóc

Product Name

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

Poziom jakości

300

Formularz

DMSO solution

rozpuszczalność

water: soluble

temp. przechowywania

−20°C

Opis ogólny

The Protease Inhibitor Cocktail for fungal and yeast extracts is a mixture of protease inhibitors in DMSO solution. The cocktail has been optimized and tested for use on fungal and yeast samples, specifically on Saccharomyces cerevisiae cells. One mL of the cocktail is recommended for the inhibition of proteases extracted from 20 g of yeast.

Specyficzność

Inhibits serine, cysteine, aspartic, and metalloproteases.

Zastosowanie

Protease Inhibitor Cocktail has been used as a component in:

FA buffer to wash spheroplasts to obtain an average DNA fragment size for chromatin immunoprecipitation (chip)
potassium phosphate buffer (pbs) to digest chitin
extraction buffer for grinding powdered mycelia for co-immunoprecipitation analysis

Cechy i korzyści

Broad specificity: inhibits a wide range of proteases, providing comprehensive protection to fungal and yeast extracts.
Tested on Saccharomyces cerevisiae cells: the cocktail has been optimized for use on this commonly studied yeast strain.
Convenient packaging: available in a 1 or 5 mL glass bottle for easy handling and storage.
Ready-to-use solution: the cocktail is supplied in DMSO solution for immediate use in protease inhibition assays.
Effective inhibition: each component in the cocktail has been carefully selected for its specific inhibitory properties, ensuring reliable and consistent results.

Komponenty

AEBSF, 100 mM
E-64, 1.4 mM
Pepstatin A, 2.2 mM
1,10-Phenanthroline, 500 mM

Inne uwagi

Mixture of protease inhibitors with broad specificity for the inhibition of serine, cysteine, aspartic and metallo-proteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), pepstatin A, E-64, and 1,10-phenanthroline.

This product is for R&D use only, not for drug, household, or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.