P7640

Phosphatase, Alkaline from bovine intestinal mucosa

Name: Phosphatase, Alkaline from bovine intestinal mucosa
Brand: SIGMA

lyophilized powder, ≥10 DEA units/mg solid

Synonim(y):

Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

CAS Number:

9001-78-9

Numer WE:

3.1.3.1 (BRENDA, IUBMB)

Numer WE:

232-631-4

Numer MDL:

MFCD00131849

Kod UNSPSC:

12352204

eCl@ss:

42010105

NACRES:

NA.54

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Pomoc techniczna

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Pozwól nam pomóc

Pomoc techniczna

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Pozwól nam pomóc

pochodzenie biologiczne

bovine intestinal mucosa

Poziom jakości

200

Formularz

lyophilized powder

aktywność właściwa

≥10 DEA units/mg solid

masa cząsteczkowa

dimer ~160 kDa

Warunki transportu

wet ice

temp. przechowywania

−20°C

Zastosowanie

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It is routinely used to dephosphorylate proteins and nucleic acids. It may be used for protein labeling when high sensitivity is required. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase.. Product P7640 has been used to study the monoclonal alkaline phosphatase-anti-alkaline phosphatase (APAAP) complex.

High specific activity grade recommended for antibody and protein conjugation.

Działania biochem./fizjol.

Alkaline phosphatase, from bovine intestinal mucosa, is most stable in the pH range 7.5-9.5. The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols and it requires zinc, and magnesium or calcium divalent ions for activity.

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Właściwości fizyczne

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.