P7457

Carboxy-terminal FLAG-BAP^™ Fusion Protein

• Numer MDL: MFCD00130921
• Kod UNSPSC: 12352202
• NACRES: NA.32

Właściwości

• Formularz: liquid
• Poziom jakości: 200
• masa cząsteczkowa: ~49 kDa
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Carboxy-terminal FLAG-BAP Fusion Protein is a 466 amino acid C-terminal FLAG fusion protein of E.coli bacterial alkaline phosphatase (BAP).

Zastosowanie

Carboxy-terminal FLAG-BAP^™ Fusion Protein has been used in the immunoprecipitation of the reporter protein in human embryonic kidney (HEK) cell lysate and as a FLAG-tagged control protein in solid-phase binding assay of spermatogenic immunoglobulin superfamily protein (SgIGSF).

Learn more product details in our FLAG^® application portal.

Działania biochem./fizjol.

The FLAG sequence comprises of the eight-amino acid sequence AspTyrLysAspAspAspAspLys and is hydrophilic. FLAG fusion proteins are expressed in bacterial, yeast and mammalian cells. FLAG epitope-tagged bacterial alkaline phosphatase is employed in immunoaffinity purification. Alkaline phosphatase based fusion protein have wide clinical applications in immunodetection, enzyme immunoassay and enzyme-linked immunosorbent assay.

Inne uwagi

Control protein

Postać fizyczna

Supplied in 10 mM Tris, 120 mM NaCl, 0.05 mM ZnCl2

Uwaga dotycząca przygotowania

Dilute the ANTI-FLAG M2 antibody solution to 10 mg/ml

Informacje prawne

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)