P6282

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt 70,000-150,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Synonim(y): PLL HBr
• CAS Number: 25988-63-0
• Numer MDL: MFCD00237230
• Kod UNSPSC: 12352202
• eCl@ss: 32160406
• Identyfikator substancji w PubChem: 24898753
• NACRES: NA.75

Właściwości

• Product Name: Poly-L-lysine hydrobromide, mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• pochodzenie biologiczne: synthetic (organic)
• Poziom jakości: 200
• sterylność: γ-irradiated
• linia produktu: BioXtra
• Formularz: lyophilized powder
• masa cząsteczkowa: 70,000-150,000
• opakowanie: pkg of 5 mg
• metody: cell culture | mammalian: suitable
• pokrycie powierzchni: 4 μg/cm²
• rozpuszczalność: H₂O: soluble 50 mL/vial, clear, colorless
• Warunki transportu: ambient
• temp. przechowywania: −20°C
• ciąg SMILES: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• Klucz InChI: WBSCNDJQPKSPII-KKUMJFAQSA-N

Opis

Opis ogólny

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Zastosowanie

Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm². Optimal conditions for attachment must be determined for each cell line and application.

Działania biochem./fizjol.

Poly-L-lysine serves as a pre-coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Komponenty

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Przestroga

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Komentarz do analizy

Molecular weight based on viscosity. Also assayed by MALLS.

Inne uwagi

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)