P2714

Protease Inhibitor Cocktail

Name: Protease Inhibitor Cocktail
Brand: SIGMA

lyophilized powder, for the inhibition of serine, cysteine and metallo-proteases, for general use, lyophilized powder

Synonim(y):

Inhibitor proteazy

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About This Item

Kod UNSPSC:

12352200

NACRES:

NA.77

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Pomoc techniczna

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Product Name

Protease Inhibitor Cocktail powder, for general use, lyophilized powder

pochodzenie biologiczne

bovine lung

Poziom jakości

300

Formularz

lyophilized powder

rozpuszczalność

water: soluble

temp. przechowywania

−20°C

Opis ogólny

The protease inhibitor cocktail has been optimized and tested for general use. It consists of water-soluble protease inhibitors that effectively inhibit serine, cysteine, and metalloproteases. This product is supplied as a lyophilized powder.

Specyficzność

Inhibits serine, cysteine, and metallo-proteases.

Zastosowanie

Protease Inhibitor Cocktail powder has been used as a component:

in radioimmunoprecipitation assay buffer to lyse the avian crystalline lens samples for western blot analysis
in phosphate buffer solution (PBS) for grinding recombinant protein expressed leaves to purify virus-like particles (VLPs)
in PBS to dissolve lyophilized skin secretion sample of A. loloensis for peptide purification
in 0.1 M NaCl solution to wash and collect skin secretions of frog

Działania biochem./fizjol.

The components of this mixture, such as AEBSF and Aprotinin act to inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein proteases.

Komponenty

This mixture contains individual components, including:

AEBSF at 2 mM
Aprotinin at 0.3 μM
Bestatin at 116 μ
E-64 at 14 μM
EDTA at 1 mM
Leupeptin at 1 μM

Each component has specific inhibitory properties.

Przestroga

The lyophilized powder is stable for 4 years when stored at -20°C.

Ilość

One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.

Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume of cocktail required.

Uwaga dotycząca przygotowania

This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.

Inne uwagi

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

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