P1860

Protease Inhibitor Cocktail

DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use in tissue culture media, DMSO solution

• Synonim(y): inhibitor proteazy
• Numer WE: 200-664-3
• Numer MDL: MFCD00677817
• Kod UNSPSC: 12352200
• NACRES: NA.77

Właściwości

• Product Name: Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
• Poziom jakości: 200
• Formularz: DMSO solution
• rozpuszczalność: water: soluble
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Protease Inhibitor Cocktail demonstrated non-toxicity towards the following cell lines after 48 hours exposure:
A431, CHO, COS, HepG2, and HeLa adherent cell lines
Jurkat and HL-60 cell lines grown in suspension

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Specyficzność

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Zastosowanie

Protease Inhibitor Cocktail has been used:

• as a supplement in supernatants and lysates for ex vivo infection experiments using human precision-cut lung slices (PCLS)
• to inhibit proteolysis by trypsin to prove that proteolysis is responsible for restoration of catalysis of corona-inhibited nanozymes
• in trypsin solution for trypsin-inhibited studies
• as a component in radioimmunoprecipitation assay (RIPA) buffer to lyse leukocytes for western blot

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Działania biochem./fizjol.

Protease inhibitor cocktail is designed for use in tissue culture media. It is recommended as an additive to tissue culture media to safeguard against the degradation of secreted proteins originating from the cultured tissue.

Cechy i korzyści

• Broad specificity
• Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.
• Contains no metal chelators: Ensures compatibility with downstream applications
• Convenient packaging: 1 mL in glass bottle
• Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Komponenty

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Przestroga

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Ilość

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Postać fizyczna

Solution in DMSO (D 2650, Hybri-Max).

Inne uwagi

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): 188.6 °F
• Temperatura zapłonu (°C): 87 °C