F1283
Fetal Bovine Serum
Fetal Bovine Serum, USA origin, Ultra Low IgG
Synonim(y):
FBS, FCS, Fetal Calf Serum
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About This Item
Kod UNSPSC:
12352207
NACRES:
NA.71
Pomoc techniczna
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Pozwól nam pomócPomoc techniczna
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Pozwól nam pomócpochodzenie biologiczne
bovine fetus
Poziom jakości
sterylność
sterile-filtered
linia produktu
FBS Select
skład
Bovine IgG, <5 μg/mL
Hemoglobin, ≤20 mg/dL
pochodzenie
USA origin
warunki przechowywania
avoid repeated freeze/thaw cycles
protect from light
metody
cell culture | mammalian: suitable
zanieczyszczenia
≤10 EU/mL endotoxin
pH
6.8-8.1
osmolalność
260-330 mOsm/kg H2O
Warunki transportu
dry ice
temp. przechowywania
−20°C
Opis ogólny
Animal serum is commonly used to supplement basal media formulations for the optimal growth of many cell types in vitro.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Fetal Bovine Serum (FBS) is the most common serum used to supplement cell culture media due to its high nutritional content. Although it is relatively low in protein, FBS is effective in promoting and sustaining growth of vertebrate mammalian and insect cells.
IgG is the most common immunoglobulin present in FBS. Elevated levels of IgG may cause problems within certain recombinant protein or monoclonal antibody purification processes. For applications where IgG may interfere or cause issues, we have depleted IgG levels in this fetal bovine serum by sequential chromatography down to a level of < 5 ug/mL, while maintaining performance equivalent to regular FBS.
Opakowanie
Packaged in sterilized, graduated plastic bottles and sealed with a tamper indicator. Bottles are immediately frozen and stored at -10 to -40 C.
Uwaga dotycząca przygotowania
The fetal blood is aseptically collected and allowed to clot under controlled conditions. After centrifugation, the raw (unfiltered) serum is decanted from the clot, pooled and immediately frozen.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Frozen raw serum is thawed under controlled conditions and then processed through a series of membrane filters in descending pore size. FBS is filtered through three 0.1 μm filters before packaging.
Komentarz do analizy
Endotoxin and hemoglobin tested
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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Certyfikaty analizy (CoA)
Lot/Batch Number
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