D7295

Mieszanina deoksynukleotydów, 10 mM

Molecular Biology Reagent

• Synonim(y): 10mM mieszanina dNTP, Mieszanka dNTP, dNTP
• Kod UNSPSC: 41106305
• NACRES: NA.52

Właściwości

• Poziom jakości: 300
• Formularz: liquid
• stężenie: 10 mM
• kolor: colorless
• Zastosowanie: agriculture
• obecność zanieczyszczeń: DNase, RNase, none detected
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

Zastosowanie

dNTP Mix has been used:

• in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
• in reverse transcription of total RNA to cDNA.
• as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
• routine and long PCR
• manual and automated DNA sequencing
• cDNA synthesis and labeling reactions

Cechy i korzyści

• Purity of each dNTP: Minimum 99%
• Conveniently formulated; 1 μL is used per 50 μL PCR
• Equimolar amounts of each dNTP means less pipetting
• Minimize risk of contamination in PCR
• UltraPure dNTPs can help maximize consistency and yields in critical PCR reactions

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable