D5648

DMEM - high glucose

With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture

• Synonim(y): DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
• Kod UNSPSC: 12352207
• NACRES: NA.75

Właściwości

• Product Name: Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
• Poziom jakości: 500
• Formularz: powder
• metody: cell culture | mammalian: suitable
• komponenty: phenol red: yes; L-glutamine: yes; NaHCO₃: no; HEPES: no; glucose: high; sodium pyruvate: no
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue

Ilość

Formulated to contain 13.4 grams of powder per liter of medium.

Rekonstytucja

Supplement with 3.7 g/L sodium bicarbonate.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable