D1501

Deoxyribonucleic acid sodium salt from calf thymus

Type I, fibers

• Synonim(y): ctDNA, DNA sodium salt from calf thymus, Thymonucleic acid sodium salt
• CAS Number: 73049-39-5
• Numer MDL: MFCD00130922
• Kod UNSPSC: 41106305
• eCl@ss: 32160414
• NACRES: NA.51

Właściwości

• typ: Type I
• Poziom jakości: 300
• Formularz: fibers
• kolor: white
• temp. przechowywania: 2-8°C

Opis

Zastosowanie

Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.

Jakość

"Highly Polymerized"

Właściwości fizyczne

λmax _{259 nm} (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.

Postać fizyczna

fibrous preparation

Uwaga dotycząca przygotowania

This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.

This product is prepared from calf thymus tissue of unspecified gender.

Rekonstytucja

Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.

This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)