CMC0016

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

• Synonim(y): Szczep BL21
• Kod UNSPSC: 41106202
• NACRES: NA.85

Właściwości

• Product Name: BL21(DE3) Electrocompetent Cells, for protein expression
• pochodzenie biologiczne: Escherichia coli
• klasa czystości: Molecular Biology
• tryb wzrostu: adherent or suspension
• morfologia: rod shaped
• metody: microbiological culture: suitable
• transformacja komórek: competent cell type: electrocompetent; transformation efficiency: ≥5 × 10⁹ cfu/μg
• Warunki transportu: dry ice
• temp. przechowywania: −70°C

Opis

Opis ogólny

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Cechy i korzyści

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 10⁹ cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Komponenty

• BL21(DE3) electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for expression

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids