C9263

Collagenase from Clostridium histolyticum

Type V, ≥1 FALGPA units/mg solid, >125 CDU/mg solid

• Synonim(y): Clostridiopeptidase A
• CAS Number: 9001-12-1
• Numer WE: 3.4.24.3 (BRENDA, IUBMB)
• Numer WE: 232-582-9
• Numer MDL: MFCD00130830
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• Poziom jakości: 200
• Formularz: powder
• aktywność właściwa: ≥1 FALGPA units/mg solid; >125 CDU/mg solid
• masa cząsteczkowa: 113-114 kDa
• rozpuszczalność: TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 1-2 mg/mL
• Zastosowanie: diagnostic assay manufacturing
• temp. przechowywania: −20°C

Opis

Opis ogólny

Clostridium histolyticum collagenases (CCH) are classified into two types ; I and II. Both are a single polypeptide with molecular weight of 114kDa - 113kDa, respectively. CCH I contains tandem domains and CCH II contain single domain for collagen binding.

Zastosowanie

Collagenase from Clostridium histolyticum has been used in a study to investigate the degradation of collagen by the cariogenic bacteria, Streptococcus mutans. Collagenase from Clostridium histolyticum has also been used in a study to investigate the survivability of collagen micronetworks in the presence of collagenase.

Collagenase has been used in the preparation of arterial tissue for the study of Advanced Glycosylation End Products (AGE). The enzyme has also been used along with other proteases for the disaggregation of human tumor, mouse kidney, human brain, and lung epithelial tissues among several others. It is also effective in liver and kidney perfusion studies, digestion of pancreas, and isolation of nonparenchymal hepatocytes. The product has been used to digest forearm skin biopsies from Parkinson disease patients to grow human skin fibroblast primary cultures. The enzyme from Sigma has been used for rodent islet isolation in a study that assayed glucokinase functions.

Działania biochem./fizjol.

Clostridium histolyticum collagenase (CCH) is a digestive enzyme acting particularly on collagen. It is useful in the treatment of Dupuytren′s contracture, fibroproliferative disorder.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Definicja jednostki

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.

Komentarz do analizy

Also contains clostripain, nonspecific neutral protease, and tryptic activities.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H334,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves