C7774

Human Collagen Type I

from human placenta, powder, suitable for cell culture

• CAS Number: 9007-34-5
• Numer WE: 232-697-4
• Numer MDL: MFCD00130825
• Kod UNSPSC: 12352202
• NACRES: NA.61

Właściwości

• Product Name: Collagen from human placenta, Bornstein and Traub Type I (Sigma Type VIII), powder
• pochodzenie biologiczne: human placenta
• Poziom jakości: 200
• Formularz: powder
• metody: cell culture | mammalian: suitable
• zanieczyszczenia: HIV, hepatitis B and hepatitis C, none detected
• numer dostępu UniProt: P02666; P02668; P53420; Q01955; Q14031
• temp. przechowywania: 2-8°C
• informacje o genach: bovine ... CSN2(281099), CSN3(281728) ; human ... COL4A3(1285), COL4A4(1286), COL4A6(1288)

Opis

Zastosowanie

This product is intended to produce thin layer coatings on tissue culture plates to facilitate attachment of anchorage-dependent cells, recommended for use at 6-10 μg/cm². It is NOT intended for production of 3-D gels. Type I collagen is often used in cell culture as an attachment substratum with myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have all been cultured successfully on films or gels of type I collagen. Collagen type I may also be used in research of Idiopathic pulmonary fibrosis (IPF), studies on the effect of ER stress IPF on lung fibroblasts. Collagen in acidic solution can produce three dimensional scaffolding with use in bioengineering and cell culture applications.

Działania biochem./fizjol.

Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues.

Komponenty

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type I collagen differs from other collagens by its low lysine hydroxylation and low carbohydrate composition.

Uwaga dotycząca przygotowania

Prepared by a modification of the pepsin extraction and salt fractionation method of Niyibizi, C., et al., J. Biol. Chem., 259, 14170 (1984). It is an acid soluble collagen that can be dissolved in water with acetic acid added to pH 3.0 (5 mg/mL), yielding an opalescent, colorless solution.

Inne uwagi

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)