C7521

Human Collagen Type IV

from human placenta, powder, suitable for substrate for collagenase and for diagnostic assay manufacturing

• Synonim(y): Collagen Type 4
• CAS Number: 9007-34-5
• Numer WE: 232-697-4
• Numer MDL: MFCD00130825
• Kod UNSPSC: 12352202
• NACRES: NA.54

Właściwości

• Product Name: Collagen from human placenta, Bornstein and Traub Type IV, powder
• pochodzenie biologiczne: human placenta
• Poziom jakości: 200
• opis: Sigma Type VI
• Formularz: powder
• zanieczyszczenia: HIV, hepatitis B and hepatitis C, none detected
• rozpuszczalność: 0.5 M acetic acid: 1 mg/mL
• przydatność: suitable for substrate for collagenase
• numer dostępu UniProt: P08572; P29400; P53420; Q01955; Q14031
• Zastosowanie: diagnostic assay manufacturing
• temp. przechowywania: −20°C
• informacje o genach: human ... COL4A2(1284), COL4A3(1285), COL4A4(1286), COL4A5(1287), COL4A6(1288)

Opis

Działania biochem./fizjol.

During development, collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells.

Komponenty

All collagen molecules are composed of three polypeptide chains arranged in a triple helical conformation, with a primary structure that is mostly a repeating motif with glycine in every third position and proline or 4-hydroxyproline frequently preceding the glycine residue. Type IV collagen occurs only in the basement membranes and contains up to six genetically distinct a-chains.

Uwaga dotycząca przygotowania

This is a lyophilized powder that can be reconstituted in sterile .5 M acetic acid, PBS or water at 1 mg/mL. A PBS solution will be stable for at least one year at -20°C. It is prepared by a modification of the pepsin extraction method of Glanville, R.W., et al.

Komentarz do analizy

An SDS polyacrylamide gel electrophoresis test run under reducing conditions consistent with basement membrane collagen yields three major bands.

Inne uwagi

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Be wary of confusing Sigma-type designations with recognized collagen classification types.

Ta strona może zawierać tekst przetłumaczony maszynowo.

Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)