C0255

Collagenase from Clostridium histolyticum

Name: Collagenase from <I>Clostridium histolyticum</I>
Brand: SIGMA

purified by chromatography, ≥500 CDU/mg solid (CDU = collagen digestion units), lyophilized powder

Synonim(y):

Clostridiopeptidase A

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About This Item

CAS Number:

9001-12-1

Numer WE:

3.4.24.3 (BRENDA, IUBMB)

Numer WE:

232-582-9

Numer MDL:

MFCD00130830

Kod UNSPSC:

12352204

NACRES:

NA.54

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Formularz

lyophilized powder

aktywność właściwa

≥500 CDU/mg solid (CDU = collagen digestion units)

masa cząsteczkowa

68-130 kDa

oczyszczone przez

chromatography

rozpuszczalność

TESCA buffer (50 mM TES, 0.36 mM Calcium chloride, pH 7.4): soluble 0.05-0.1 mg/mL at 37 °C

obecność zanieczyszczeń

Clostripain ≤2.0 U/mg

temp. przechowywania

2-8°C

Opis ogólny

Collagenase from Clostridium histolyticum comprises of collagenases G and H. Collagenase has N-terminal activator domain and C-terminal peptidase domain. The collagen binding domains (CBD) and polycystic kidney disease-like (PKD-like) domain resides in the peptidase domain. The collagenase module undergoes structural changes upon calcium binding.

Zastosowanie

Collagenase from Clostridium histolyticum has been used:

for the recovery of neutrophils from collagen gels
for the dissociation of breast tumor biopsies into single cells
for the isolation of primary human endometrial stromal cells(HESC) from endometrial biopsies

Collagenase from Clostridium histolyticum may be used for the isolation of cells from many types of animal tissue. It may be used for the disaggregation of human tumor, mouse kidney, human adult/fetal brain, lung and many other tissues. It may also be used in liver and kidney perfusion studies, for the digestion of pancreas, isolation of nonparenchymal rat liver cells and for hepatocyte preparation.

Działania biochem./fizjol.

Collagenase is activated by four gram atom calcium per mole enzyme. It is inhibited by ethylene glycol-bis(beta-aminoethyl ether) - N, N, N′,N′-tetraacetic acid, beta-mercaptoethanol, glutathione, thioglycolic acid and 8-hydroxyquinoline.

Crude collagenases are mixtures of enzymes (mostly proteases) secreted by C. histolyticum. Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four gram atom calcium (Ca²⁺) per mole enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa.

Opakowanie

Product sold on the basis of collagen digestion units (CDU).

Definicja jednostki

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C in the presence of calcium ions. One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at 25°C. One Neutral Protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole of tyrosine per 5 hr at pH 7.5 at 37°C. One Clostripain Unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25°C in the presence of DTT.