PPB006

Phosphate buffered saline

powder, pH 7.4, pHast Pack^™

• Synonim(y): 1X bufor płuczący PBS
• Numer MDL: MFCD00131855
• Kod UNSPSC: 12161700
• NACRES: NA.21

Właściwości

• Product Name: Phosphate buffered saline, pH 7.4, pHast Pack^™, powder
• linia produktu: pHast Pack^™
• Formularz: powder
• warunki przechowywania: (Tightly closed. Dry)
• zanieczyszczenia: <5—2 ppm Heavy metals (Lead)
• kolor: white
• pH: 7.4
• rozpuszczalność: soluble, clear, colorless
• ślady kationów: Fe: <10 ppm
• obecność zanieczyszczeń: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• temp. przechowywania: room temp

Opis

Opis ogólny

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Zastosowanie

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

Cechy i korzyści

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Opakowanie

Foil pouches

Rekonstytucja

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Przechowywanie i stabilność

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Inne uwagi

For additional information on our range of Biochemicals, please complete this form.

Informacje prawne

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable