88793

Atto 532 NHS ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Synonym(s): Atto 532
• MDL number: MFCD05865401
• UNSPSC Code: 12352108
• NACRES: NA.32

Properties

• product line: BioReagent
• Quality Level: 100
• Assay: ≥90% (HPLC); ≥90% (degree of coupling)
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• λ: in methanol: water (1:1) (with 0.1% perchloric acid)
• UV absorption: λ: 532-538 nm A_max
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 532 NHS ester is a fluorescent dye related to the well-known laser dye, Rhodamine 6G. The fluorescence activity is excited efficiently at the 515-545nm range. A suitable excitation source for Atto 532 is the 532 nm output of the frequency-doubled Nd: YAG laser.

Application

Atto 532 NHS ester is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, and STED. In addition, the dye is used in flow cytometry (FACS) and fluorescence in-situ hybridization (FISH) methods.

Features and Benefits

Characteristic features of the Atto 532 NHS ester are:

• Strong Absorption.
• High Fluorescence quantum yield.
• High Photostability.
• Excellent water solubility.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves