82452

Proteinase K, immobilized on Eupergit^® C from Tritirachium album

powder (granular), ≥1500 U/g

• Synonym(s): Endopeptidase K, immobilized on Eupergit^® C
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (Tritirachium album)
• form: powder (granular)
• specific activity: ≥1500 U/g
• storage temp.: 2-8°C

Description

General description

Proteinase K is secreted extracellular medium by mold, Tritirachium album. It hydrolyses keratin and shows substrate specificity similar to serine alkaline proteinases.

Application

Proteinase K, immobilized on Eupergit^® C from Tritirachium album has been used:

• in the protolysis of human serum
• in the digestion of gruel samples for enzyme linked immune assays (ELISA)
• in proteolytic stability studies of calcium ion flux inducers

Biochem/physiol Actions

Proteinase K hydrolyses keratin by cleaving peptide bonds adjacent to the carboxyl group of aliphatic and aromatic amino acids.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol N-acetyl-L-tyrosine-ethylester (ATEE) per minute at pH 9.0 and 30 °C.

Legal Information

Eupergit is a registered trademark of Röhm GmbH & Co. KG

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Statements: H315,H319,H334,H335
• Precautionary Statements: P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves