H9394

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): HBSS
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: NaHCO₃: 0.35 g/L; glucose: 1.0 g/L (Dextro); phenol red: 0.011 g/L
• shipped in: ambient

Description

General description

Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem.

Application

Hanks′ Balanced Salt solution has been used:

• as a component of the culture medium for culturing of mouse cortical slices
• as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs)
• for in situ perfusions of mice liver

Biochem/physiol Actions

Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable