I6529

IMDM

With sodium bicarbonate and ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
• MDL number: MFCD00217469
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: Iscove′s Modified Dulbecco′s Medium, With sodium bicarbonate and L-glutamine, liquid, sterile-filtered, suitable for cell culture, suitable for hybridoma
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: glucose: yes; phenol red: yes; NaHCO₃: yes; L-glutamine: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

Iscove′s Modified Dulbecco′s Medium contains selenium, additional amino acids and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate, and is a modification of Dulbecco′s Modified Eagle′s medium (DME). Iscove′s medium can be used to support murine B lymphocytes, bone marrow hemopoietic tissue, B cells stimulated with lipopolysaccharide, T lymphocytes, and various hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture:

• human hepatocellular carcinoma (HCC) cell line HepG2 as monolayers
• to culture synovial tissue samples
• bacterial strain inoculum for stimulation of immune cell experiments

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable