I3390

IMDM

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonym(s): Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM
• MDL number: MFCD00217469
• UNSPSC Code: 12352207
• NACRES: NA.75

Properties

• Product Name: Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma
• Quality Level: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | hybridoma: suitable; cell culture | mammalian: suitable
• impurities: endotoxin, tested
• components: phenol red: yes; L-glutamine: no; glucose: yes
• shipped in: ambient
• storage temp.: 2-8°C

Description

Application

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Reconstitution

Supplement with 0.584 g/L L-glutamine

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable