SML3580

Rapa*-3, mixture of E and Z isomers

≥98% (HPLC)

• Synonym(s): 27-(O-Ethyloxime)-rapamycin, Rapa 3(E/Z), Rapa-3(E/Z), Rapa3(E/Z)
• Empirical Formula (Hill Notation): C₅₃H₈₄N₂O₁₃
• Molecular Weight: 957.24
• UNSPSC Code: 12352200
• NACRES: NA.21

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: powder
• color: white to beige
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: -10 to -25°C
• SMILES string: O=C1O[C@]([C@@H](C)C[C@H]2CC[C@H](O)[C@@H](OC)C2)([H])CC([C@@H](C)/C=C([C@H](O)[C@H](OC)C([C@@H](C)C[C@@H](C)/C=C/C=C/C=C([C@H](OC)C[C@]3([H])O[C@@](O)([C@@H](C)CC3)C(C(N4CCCC[C@]14[H])=O)=O)\C)=O)\C)=NOCC

Description

Biochem/physiol Actions

Rapa*-3Z is a rapamycin analog that selectively targets FKBP12 with V55G mutation, while exhibiting reduced affinity toward the wild-type FKBP12 (mTOR FRB association induction EC50 = 11.8 nM/V55G vs 639 nM/wt FKBP12). Rapa*-3Z selectively inhibits the growth, the phosphorylation level of mTORC1 downstream pS6K/pS6 and mTORC2 downstream pAkt in 293 transfectants experssing the V55G FKBP12 mutant (mutant cells pS6K/pS6 IC50 = 1.0/1.3 nM vs. 76/121 nM in wt cells). Rapa*-3Z potently inhibits the larval development of transgenic flies that express human FKBP12 V55G (by 70%/75 μM and 100%/100 μM), but not those with wild-type human FKBP12.

Rapamycin analog that selectively inhibits mTOR signaling in FKBP12(V55G) mutant-expressing cells and organisms.

Safety Information

• Pictograms: GHS08
• Signal Word: Warning
• Hazard Statements: H351,H361fd
• Precautionary Statements: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable