MC116

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514

Properties

• biological source: human lymph
• packaging: tube of 5 μg 90110501-DNA-5UG; pkg of vial of cells 90110501-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Lymphoblast
• products: Surface Immunoglobulin M (IgM), pentameric Immunoglobulin M (IgM)
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human lymphoma

Cell Line Description

Derived from the pleural effusion of a patient with undifferentiated lymphoma. The cells are EBNA negative, express surface IgM Lambda and secrete pentameric IgM which does not bind to protein A. Complement and Fc receptors were not detected.

Application

Hormone and tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 12
D5S818: 12
D7S820: 10,13
THO1: 9.3,10
TPOX: 8,10
vWA: 15,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C. A maximum cell density of 2-4x1,000,000 cells/ml is achieved.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.