C6423

α-Chymotrypsin from bovine pancreas

suitable for protein sequencing, salt-free, lyophilized powder

• CAS Number: 9004-07-3
• Número CE: 3.4.21.1 (BRENDA, IUBMB)
• Número CE: 232-671-2
• Número MDL: MFCD00130481
• Código UNSPSC: 12352204
• eCl@ss: 42010112
• NACRES: NA.26

Propiedades

• grado: Proteomics Grade
• Nivel de calidad: 200
• Formulario: salt-free, lyophilized powder
• mol peso: 25 kDa
• idoneidad: suitable for protein sequencing
• Nº de acceso UniProt: P00767
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: cow ... CTRB1(618826)

Descripción

Descripción general

α-Chymotrypsin from bovine pancreas (bovine pancreatic α-chymotrypsin, CHT) is an enzyme protein. The influence of varying concentrations of organic solvents like ethanol, 1,4-dioxane and acetonitrile on CHT has been reported.

Chymotrypsin (Chy) is a serine protease. It corresponds to a molecular weight of 25.7 kDa and is widely used in pharmaceutical industry. It is synthesized in pancreas from chymotrypsinogen and require calcium for this conversion.

Aplicación

α-Chymotrypsin from bovine pancreas is used for the following applications:

• Protein Identification by mass spectrometry (MS)
• The isolation and characterization of myosin heavy chains
• Toxin preparation
• The incubation of infected and uninfected cells for analysis of cellular proteins by SDS-PAGE

α-Chymotrypsin from bovine pancreas (BPC) may be used as a catalyst in the preparation of tetrahydroquinoline derivatives by Povarov reaction.

Acciones bioquímicas o fisiológicas

α-Chymotrypsin from bovine pancreas is stabilized by Ca²⁺ and catalyzes the hydrolysis of peptide bonds in particular the amino acids tyrosine, phenylalanine, tryptophan, and leucine at their C-terminal side. α-Chymotrypsin is inhibited by Cu²⁺ and Hg.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definición de unidad

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Otras notas

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Pictogramas: GHS08,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H302,H315,H319,H334,H335,H400
• Consejos de prudencia: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves