C4129

α-Chymotrypsin from bovine pancreas

Type II, lyophilized powder, ≥40 units/mg protein

• Sinónimos: α-chymotrypsin A and B, alpha-chymotrypsin
• CAS Number: 9004-07-3
• Número CE: 3.4.21.1 (BRENDA, IUBMB)
• Número CE: 232-671-2
• Número MDL: MFCD00130481
• Código UNSPSC: 12352204
• eCl@ss: 42010112
• NACRES: NA.54

Propiedades

• tpo: Type II
• Nivel de calidad: 300
• Formulario: lyophilized powder
• actividad específica: ≥40 units/mg protein
• mol peso: 25 kDa
• composición: protein, ≥85%
• color: white to off-white
• Nº de acceso UniProt: P00767
• aplicaciones: diagnostic assay manufacturing
• temp. de almacenamiento: −20°C
• Información sobre el gen: cow ... CTRB1(618826)

Descripción

Aplicación

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Acciones bioquímicas o fisiológicas

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Definición de unidad

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Nota de preparación

Produced from 3× crystallized chymotrypsinogen

Nota de análisis

Protein determined by E1%/280

Otras notas

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

Información de seguridad

• Pictogramas: GHS08,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H302,H315,H319,H334,H335,H400
• Consejos de prudencia: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Faceshields, Gloves