PPB006

Disolución salina tamponada con fosfato

powder, pH 7.4, pHast Pack^™

• Sinónimos: PBS
• Número MDL: MFCD00131855
• Código UNSPSC: 12161700
• NACRES: NA.21

Propiedades

• Product Name: Disolución salina tamponada con fosfato, pH 7.4, pHast Pack^™, powder
• Línea del producto: pHast Pack^™
• Formulario: powder
• condiciones de almacenamiento: (Tightly closed. Dry)
• impurezas: <5—2 ppm Heavy metals (Lead)
• color: white
• pH: 7.4
• solubilidad: soluble, clear, colorless
• trazas de catión: Fe: <10 ppm
• actividad extraña: DNAse, none detected; Nickase, none detected; Protease, none detected; RNAse, none detected
• temp. de almacenamiento: room temp

Descripción

Descripción general

Phosphate buffered saline or PBS, pH 7.4 is a commonly used biological buffer in molecular biology. A 1X working solution is typically used in cell culture and electrophoresis assays to wash away non-adherent cells or any unbound biomolecules. PBS is hypo-osmotic and can be harmful to cells, causing swelling and resulting in cell lysis, if left for extended periods.

Aplicación

Phosphate buffered saline is commonly used in washing solutions for:

• ELISA
• Western Blotting and other protein applications
• Cell culture (sterile filtration recommended)
• Resuspension and sonication of cells
• Immunohistochemistry

Características y beneficios

• Save time with our ready-to-use formulation
• No calculations, measurements, and adjusting pH
• Reliable quality for reproducibility
• Suitable for use in cell culture and electrophoresis
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Envase

Foil pouches

Reconstitución

Contents of one pouch, when dissolved in 500mL of distilled or deionized water, will yield a 1X solution containing 10mM Na2HPO4, 1.8mM KH2PO4, 138mM NaCl, 2.7mM KCl, pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free. Certified for use in cell culture.

Almacenamiento y estabilidad

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Otras notas

For additional information on our range of Biochemicals, please complete this form.

Información legal

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable