93595

Azul de tripano solution

0.4%

• Fórmula empírica (notación de Hill): C₃₄H₂₄N₆Na₄O₁₄S₄
• CAS Number: 72-57-1
• Peso molecular: 960.81
• Beilstein: 4360496
• Número MDL: MFCD00003969
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 329770254
• NACRES: MA.02

Propiedades

• Formulario: liquid
• Nivel de calidad: 200
• caducidad: limited shelf life, expiry date on the label
• concentración: 0.4%
• técnicas: microbe id | staining: suitable
• color: blue to very dark blue
• densidad: 1.007 g/mL at 20 °C
• εmax: 1.4 at 603 nm in methanol
• idoneidad: suitable for microscopy
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4
• InChI: 1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
• Clave InChI: GLNADSQYFUSGOU-UHFFFAOYSA-J

Descripción

Descripción general

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Aplicación

Trypan blue applications include:

• it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
• employed as a collagen stain in a Van Gieson procedure
• To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
• used as a fluorescent tracer of cell populations in embryology
• used as a tumor promoter modulating permeability of lysosomal membranes

Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Acciones bioquímicas o fisiológicas

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Forma física

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H350
• Consejos de prudencia: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Clasificaciones de peligro: Carc. 1B
• Código de clase de almacenamiento: 6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type ABEK (EN14387) respirator filter